IMPRS-gBGC workshop 'Analytical Techniques'

Category: Skill course
0.2 CP per course day

This skill course provides insights into different analytical techniques which are applied to resolve key questions in global biogeochemistry and related earth system sciences. Please do not participate in a course module if you are already familiar with the technique. The modules will give introductions to the methods. They do not address users.

1. Overview

Date: September/October, 2016
Locations:
- MPI-BGC, Hans-Knöll-Str. 10
- Institute for Inorganic and Analytical Chemistry (IAAC), seminar room #127, Lessingstr. 8
- Institute of Photonic Technology (IPHT), Albert-Einstein-Straße 9
- Institute of Geosciences (IGW), Burgweg 11
- Institute of Microbiology, Neugasse 25
- Trace Gas Analysis and Gas Standard Preparation Flask and Calibration Laboratory (CAL-FCL), Kahlaische Str. 4

2. Agenda

Legend
L = lecture, S = seminar, D = demonstration, P = Practical

Day & Time	Type	Content	Who & Where
Tue, Sept 13		Analytical Techniques in Microbiology	Katrin Krause (Microbiology, FSU Jena)
9.00-10.00 am	L	Introduction fungi and fluorescence microscopy	Seminar room (Neugasse 25)
10.00-12.00 am	P	(Immuno)fluorescence staining	Laboratory (Neugasse 25)
1.00-4.00 pm	D, P	Fluorescence and laserscanning	Laboratory (Neugasse 25)
Thu, Sept 15		Routine Measurements and Analysis	Ines Hilke, Sonja Rosenlöcher, Birgit Fröhlich (MPI-BGC)
9.00-10.30 am	L	How to determine carbon, nitrogen, and other parameters in solid and liquid samples? overview of analytical methods (e.g. elemental analysis, sum parameter analysis, flow injection analysis, and ion chromatography) no limits? - instrumentation, pro and cons of application	B0.002 (MPI-BGC)
11.00-12.00 am	D	Demonstration of analytical methods and laboratory instruments	A1.019 (MPI-BGC)
13.00-16.00 pm	P	Analytics of solid and liquid sample	A1.019 (MPI-BGC)
Fri, Sept 16		Volatile organic compounds	Georg Pohnert (IAAC, FSU)
9:00-11.00 am	L	"Headspace SPME, GC/MS" Basics, enrichment of compounds from the gaseous phase → analysis	Seminar room #127 (Lessingstr. 8)
11.00 am - 1.00 pm	P	Lab class "DMS from water samples" as an example + discussion & transfer of results (group 1)	Lab (Lessingstr. 8)
1.00-3.00 pm	P	Lab class "DMS from water samples" as an example + discussion & transfer of results (group 2)	Lab (Lessingstr. 8)
Mon, Sept 19		LA-ICP-MS	Neele van Laaten (FSU Jena, IGW)
9.00-11.00 am	L	Theory of LA-ICP-MS	Seminar room (IGW)
11.00-12.00 am	D	Practical applications of LA-ICP-MS	Lab (IGW)
Wed, Sept 21		Principles of Good Laboratory Praxis	Dr. Michael Raessler (MPI-BGC)
9.00-11.00 am	S	Lab organisation Documentation starting from arrival of samples Helpful advice for 'customers' of analytical facilities	B0.002 (MPI-BGC)
Wed, Sept 28		Extraction methods and associated techniques	Molecular Biogeochemistry group (MPI-BGC)
9.00-12.00 am & 1.00-4.00 pm	L, D	Extraction Column and gas chromatography liquid/liquid extraction solid phase extraction distribution ratio selectivity reading chromatograms Recovery rate Calibration Quantification of alkanes	B0.004 (MPI-BGC)
Sept 29-30		Raman spectroscopy	Torsten Frosch (FSU Jena / IPHT)
Thu, Sept 29 10-12 am	L	Raman spectroscopy	B0.004 (MPI-BGC)
Thu, Sept 29 1-4 pm	D, P	Lab class: Raman gas spectroscopy	Lab (IPHT)
Fri, Sept 30 1-4 pm	D, P	Lab class: Raman microspectroscopy of minerals	Lab (IPHT)
Tue, Oct 4		GasLab	Armin Jordan & Daniel Rzesanke (MPI-BGC)
9.00-11.30 am	L	Principles of trace gas measuring techniques Spectroscopy: laser-optical gas analysers, FTIR, NDIR Chromatography: GC Requirements for high-precision trace gas measurements (Standardisation, quality control) Sampling —> Laboratory analyses Critical consideration of interferences	B0.002 (MPI-BGC)
12.30-13.30 pm	D	Tour through the Trace Gas Analysis and Gas Standard Preparation Flask and Calibration Laboratory	CAL-FCL (Kahlaische Str. 4)
Tue, Oct 11		¹⁴C Analytics	Axel Steinhof (MPI-BGC)
9:15-10:30 am	L	Introduction to Accelerator Mass Spectrometry (AMS)	B0.002 (MPI-BGC)
10:30-11:00 am		Coffee break	foyer, ground floor (MPI-BGC)
11:00-11:30 am	D	Lab demonstration	C01.005 (MPI-BGC)

3. Material

Presentation of Katrin Krause: Analytical techniques in microbiology
Presentation of Ines Hilke: Routine measurements and analytics
Presentation of Georg Pohnert: Volatile organic compounds
Presentation of Neele van Laaten: LA-ICP-MS
Presentation of Dr. Rässler: Good laboratory praxis
Handout of Vanessa-Nina Roth: Extraction methods and associated techniques
Presentation of Torsten Frosch: Raman spectroscopy - will be uploaded soon
Presentation of Armin Jordan & Daniel Rzesanke: Introduction
Presentation of Armin Jordan & Daniel Rzesanke: Trace gas measurements
Presentation of Axel Steinhof: ¹⁴C Analytics

4. Feedback

The survey results are presented here. Statistics and statements should not be taken as an exhaustive or exclusive list.